EPA Method 1002


EPA Method 1002 does not directly match ALS - Columbia's list of currently available methods. Please call for availability.


Method information displayed is provided for informational purposes only. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project). Please contact us for assistance. Analytical testing dots

PDF IconView Actual EPA Method 1002 (PDF File)


EPA Method 1002:
Survival and Reproduction Test using Daphnids. Official Name: EPA Test Method 1002.0: Daphnid, Ceriodaphnia dubia, Survival and Reproduction Test

Summary:
Ceriodaphnia dubia are exposed in a static renewal system to different concentrations of effluent, or to receiving water, until 60% or more of surviving control females have three broods of offspring. Test results are based on survival and reproduction. If the test is conducted as described, the surviving control organisms should produce 15 or more young in three broods. If these criteria are met at the end of 8 days, the test must be repeated.

Scope:
This method measures the chronic toxicity of effluents and receiving waters to the daphnid, Ceriodaphnia dubia, using less than 24h old neonates during a three-brood (seven-day), static renewal test. The effects include the synergistic, atagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test organisms.

Citation:
Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

Interferences:
Introduced toxic substances; Low D.O.; High concentrations of suspended and/or dissolved solids; Pathogenic and/or predatory organisms

QC Requirements:
Reference toxicant testing with control charts for each combination of toxicant, test species, test condition, and endpoint.

Maximum Holding Time:
36h

Media:
WATER

Subcategory:
Chronic Toxicity

Concentration:
Effluents: 5 and a control Receiving waters: 100% receiving water (or minimum of 5) and a control (recommended)

Sample Prep:
Filtration, Dechlorination.

Precision:
It is a laboratory's responsibility to demonstrate its ability to obtain consistent, precise results with reference toxicants before it performs toxicity tests with effluents for permit compliance purposes. To meet this requirement, the intra-laboratory precision, expressed as percent coefficient of variation, of each type of test to be used in a laboratory should be determined by performing five or more tests with different batches of test organisms using the same reference toxicant, at the same concentrations, with the same test conditions, and the same data analysis methods.

Detection:
N/A

Revision Number:
Fourth Edition, October 2002

Analytical testing dots


Examples:  8260
TO-15

Examples:  Dioxin
Mercury
Analytical testing dots


<-- Search All Test Methods



Suggestions? The test methods page continues to expand and improve. If you have suggestions for improvement, we would enjoy hearing from you. Please contact the webmaster here.


Analytical testing dots