EPA Method 1003

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EPA Method 1003:
Green Algae, Selenastrum capricornutum, Growth Test.. Official Name: EPA Test Method 1003.0: Green Algae, Selenastrum capricornutum, Growth Test Method.

A green alga, Selenastrum capricornutum, population is exposed in a static system to a series of concentrations of effluent, or to receiving water, for 96h. The response of the population is measured in terms of changes in cell density (cell counts per mL), biomass, chlorophyll content, or absorbance.

This method measures the chronic toxicity of effluents and receiving water to the freshwater green alga, Selenastrum capricornutum, in a four-day static test. The effects include the synergistic, atagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test organisms.

Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

Introduced toxic substances; Low D.O.; High concentrations of suspended and/or dissolved solids; Pathogenic and/or predatory organisms; Nutrients

QC Requirements:
Reference toxicant testing with control charts for each combination of toxicant, test species, test condition, and endpoint.

Maximum Holding Time:


Chronic Toxicity

Effluents: 5 and a control (required minimum) Receiving waters: 100% receiving water (or minimum of 5) and a control (recommended)

Sample Prep:
Filtration, dechlorination.


Test Method 1003.0

Revision Number:
Fourth Edition, October 2002

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Examples:  8260

Examples:  Dioxin
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