EPA Method 100.2

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EPA Method 100.2:
Asbestos in Water by TEM. Official Name: Determination of Asbestos Structures Over 10 um in Length in Drinking Water

Known aliquots of a sample are filtered through a 0.1 to 0.22 um pore mixed cellulose ester (MCE). A carbon extraction replica is prepared from a portion of the filter and is examined in the TEM at a magnification of 10,000 to 20,000X. Asbestos structures are identified by morphology, selected area electron diffraction (SAED) and energy dispersive x-ray analysis (EDXA). Structures are then classified.

This method determines the presence and quantitation of asbestos structures in drinking water samples. This method allows for the quantitation of structures greater than 10 um in length.

Determination of Asbestos Structures Over 10 um in Length in Drinking Water (EPA/600R-94/134)

The following minerals exhibit morphological, chemical or crystal structure similarities similar to the asbestos minerals: Antigorite, Attapulgite (Palygorskite), Halloysite, Horneblende, Pyroxenes, Sepiolite, Vermiculite scrolls.

QC Requirements:
The quality control checks generally follow those specified in the Federal Register for AHERA analysis of air samples, and the NISTIR document relating to airborne asbestos analysis. Requirements are specified in the method, and include: sample receiving checks, sample prep checks (supplies and reagents, grid opening size, clean air monitoring, lab blank, etcher blank, grids/sample), sample analysis checks(system check, alignment check, mag calibration, camera constant, EDS line), performance checks (lab blanks, replicates, duplicates, standards, SRM, data entry, SAED patterns), and calculation checks.

Maximum Holding Time:
Filter within 48 hours of collection.



See method.


A sensitivity of 200,000 fibers per liter (0.2 MFL) is generally required.

Revision Number:

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Examples:  8260

Examples:  Dioxin
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