EPA Method 1120


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EPA Method 1120:
DERMAL CORROSION

Summary:
The assay system is an in vitro test method which is composed of two components, a synthetic macromolecular biobarrier and a Chemical Detection System (CDS). Test samples are applied on top of the macromolecular biobarrier. Corrosive samples are able to disrupt the macromolecular structure of the biobarrier. A color change in the CDS, located beneath the biobarrier, is detected visually and indicates that the test sample has altered the biobarrier sufficiently to allow its passage through the full thickness of the biobarrier. The time it takes a sample to disrupt the biobarrier is inversely proportional to the degree of corrosivity of the sample - the longer it takes to observe a color change, the less corrosive the substance is. Noncorrosive samples do not disrupt the biobarrier, or disrupt the biobarrier after a predetermined time period.

Scope:
The dermal corrosion assay system is an in vitro test method which determines the corrosive potential of a substance toward human skin. The method is simple, rapid, accurate, and may be applied to both solids, liquids and emulsions. The liquids may be aqueous or non-aqueous. Solids can be water-soluble or non-soluble. The samples may be pure chemicals, dilutions, formulations, or waste. No prior treatment of the sample is required. This method may be used to meet certain regulatory applications, e.g., DOT corrosivity measurement for Packing Groups, but is not required for determining if a waste passes or fails the characteristic of corrosivity per the RCRA definition.

Report Number:
SW-846 Ch 6

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Examples:  8260
TO-15

Examples:  Dioxin
Mercury
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