EPA Method 1632

ALS - Columbia offers EPA Method 1632 testing at these laboratories:

Kelso Kelso, Washington Laboratory

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EPA Method 1632:
Chemical Speciation of Arsenic in Water and Tissue. Official Name: Chemical Speciation of Arsenic in Water and Tissue by Hydride Generation Quartz Furnace Atomic Absorption Spectrometry.

After collection, an aliquot of water sample or tissue digestate is placed in a specially designed reaction vessel to which 6M HCl is added, followed by the addition of 4% NaBH4. Arsines are purged from the sample onto a cooled glass trap where they are then thermally desorbed into an inert gas stream that carries them into an atomic absorption spectrophotometer for detection.

This method determines inorganic arsenic (IA), arsenite (Ar+3), arsenate (As+5), monomethylarsonic acid (MMA), and dimethylarsinic (DMA) in filtered and unfiltered water and in tissue.

EPA Method Guidance CD-ROM (includes MCAWW Methods, and most current EPA Methods)

(A) Glassware contamination: Thoroughly clean glassware prior to use.(B) Reagent contamination: Use high quality reagents.(C) Contamination by carryover: May occur when a sample containing low concentrations of As is processed immediately after a sample containing relatively high concentrations of As. Rinse sample introduction system between samples with a dilute acid and reagent water.(D) Contamination by samples: Samples containing high concentrations should not be permitted into the clean room and laboratory dedicated for processing trace metal samples.(E) Contamination by indirect contact: Every piece of apparatus directly or indirectly used in the collection, processing, and analysis of water and tissue samples should be thoroughly cleaned.(F) Contamination by airborne particulate matter: Sample processing and analysis should occur as far as possible from sources of airborne contamination such as dust, dirt, particles, vapors, corroded or rusted pipes, wires, or metal-containing paint.(G) Water vapor may condense in the transfer line if it is not well heated, interfering with the determination of DMA.

QC Requirements:
The minimum requirements include an initial demonstration of laboratory capability, analysis of samples spiked with As and/or As species to evaluate and document data quality, and analysis of standards and blanks as tests of continued performance.

Maximum Holding Time:
28 days (aqueous samples).2 years (tissue samples).



0.01-50 ug/L in water.0.10-500 ug/g dry weight of tissue.

Sample Prep:


MDL determined by the procedure in 40 CFR Part 136, Appendix B.

Revision Number:
Revision A, August 1998

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Examples:  8260

Examples:  Dioxin
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