EPA Method 335.4

ALS - Columbia offers EPA Method 335.4 testing at these laboratories:

Jacksonville Jacksonville, Florida Laboratory
Kelso Kelso, Washington Laboratory
Rochester Rochester, New York Laboratory

Method information displayed is provided for informational purposes only. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project). Please contact us for assistance. Analytical testing dots

PDF IconView Actual EPA Method 335.4 (PDF File)

EPA Method 335.4:
Cyanide, total, by Colorimetry. Official Name: Determination of Total Cyanide by Semi-Automated Colorimetry

The cyanide as hydrocyanic acid (HCN) is released from cyanide complexes by means of a reflux-distillation operation and is absorbed in a scrubber containing sodium hydroxide solution. The cyanide ion is determined using an automated colorimeter with pyridine-barbituric acid chemistry.

This method is determines cyanide in drinking, surface and saline waters; and domestic and industrial wastes.

Methods for the Determination of Inorganic Substances in Environmental Samples (EPA/600/R-93/100)

(1) Oxidizing agents can destroy cyanides during storage.(2) Sulfide can complex with cyanide in sample or distillate.(3) Fatty acids cause interference during distillation.(4) Carbonate causes interference during distillation.(5) Aldehydes cause interference during distillation.(6) Nitrate/Nitrate causes interference during distillation (they can generate cyanides when combined with certain organic compounds).(7) Sulfur-containing compound causes interference during distillation by forming free sulfide that is captured in distillate.

QC Requirements:
The minimum requirements consist of an initial demonstration of laboratory capability, the periodic analysis of laboratory reagent blanks, fortified blanks, and other laboratory solutions as a continuing check on performance.

Maximum Holding Time:
14 days



0.005 to 0.5 mg/L

Data are available in the method.


Revision Number:
Revision 1.0, August 1993

Test Description:
Total Cyanide by Sem

Report Number:

Instrument used for this test:

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At least 1 analytes can be tested by ALS - Columbia using EPA Method 335.4. Please contact us for all available analytes which can be tested by EPA Method 335.4.

AnalyteCAS Number

Examples:  8260

Examples:  Dioxin
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