EPA Method 351.2


ALS - Columbia offers EPA Method 351.2 testing at these laboratories:

Jacksonville Jacksonville, Florida Laboratory
Kelso Kelso, Washington Laboratory
Rochester Rochester, New York Laboratory

Method information displayed is provided for informational purposes only. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project). Please contact us for assistance. Analytical testing dots

PDF IconView Actual EPA Method 351.2 (PDF File)


EPA Method 351.2:
Total Kjeldahl nitrogen in water by semiautomated colorimetry. Official Name: Method 351.2: Determination of Total Kjeldahl nitrogen by semi-automated colorimetry

Summary:
The sample is heated in the presence of sulfuric acid, H2SO4 for two and one half hours.The residue is cooled, diluted to 25 mL and analyzed for ammonia.This digested sample may also be used for phosphorus determination.Total Kjeldahl nitrogen is the sum of free-ammonia and organic nitrogen compounds which are converted to ammonium sulfate (NH4)2SO4, under the conditions of digestion described.Organic Kjeldahl nitrogen is the difference obtained by subtracting the free ammonia value from the total Kjeldahl nitrogen value.Reduced volume versions of this method that use the same reagents and molar ratios are acceptable provided they meet the quality control and performance requirements stated in the method.

Scope:
This method covers the determination of total Kjeldahl nitrogen in drinking, ground, and surface waters, domestic and industrial wastes.The procedure converts nitrogen components of biological origin such as amino acids, proteins and peptides to ammonia, but may not convert the nitrogenous compounds of some industrial wastes such as amines, nitro compounds, hydrazones, oximes, semicarbazones and some refractory tertiary amines.

Citation:
Methods for the Chemical Analysis of Water and Wastes (MCAWW) (EPA/600/4-79/020)

Interferences:
High nitrate concentrations (10X or more than the TKN level) result in low TKN values.If interference is suspected, samples should be diluted and reanalyzed.Method interferences may be caused by contaminants in the reagent water, reagents, glassware, and other sample processing apparatus that bias analyte response.

QC Requirements:
Demonstration of laboratory capability, and periodic analysis of laboratory reagent blanks, fortified blanks, and other laboratory solutions as a continuing check on performance.

Maximum Holding Time:
28 days

Media:
WATER

Subcategory:
Inorganic

Concentration:
0.1-20

Precision:
Precision and accuracy values were calculated using interlaboratory data from performance evaluation studies.

Detection:
MDL is established using reagent water fortified at a concentration of 2 to 3 times the estimated instrument detection limit and is determined from 7 replicate aliquots of the fortified reagent water and processed through the entire analytical method.

Revision Number:
Revision 2.0, August 1993

Test Description:
Nitrogen, Kjeldahl,

Report Number:
600/4-79-020

Instrument used for this test:
Discrete-FIA

Analytical testing dots
At least 1 analytes can be tested by ALS - Columbia using EPA Method 351.2. Please contact us for all available analytes which can be tested by EPA Method 351.2.

AnalyteCAS Number
Nitrogen7727-37-9





Examples:  8260
TO-15

Examples:  Dioxin
Mercury
Analytical testing dots


<-- Search All Test Methods



Suggestions? The test methods page continues to expand and improve. If you have suggestions for improvement, we would enjoy hearing from you. Please contact the webmaster here.


Analytical testing dots