EPA Method 353.2

ALS - Columbia offers EPA Method 353.2 testing at these laboratories:

Jacksonville Jacksonville, Florida Laboratory
Kelso Kelso, Washington Laboratory
Rochester Rochester, New York Laboratory

ALS - Columbia offers the following EPA Method 353.2 variations:

EPA Method 353.2M testing is available at the Kelso Laboratory.

Method information displayed is provided for informational purposes only. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project). Please contact us for assistance. Analytical testing dots

PDF IconView Actual EPA Method 353.2 (PDF File)

EPA Method 353.2:
Nitrate-Nitrite Nitrogen by Colorimetry. Official Name: Nitrogen, Nitrate-Nitrite (Colorimetric, Automated, Cadmium Reduction)

A filtered sample is passed through a column containing granulated copper-cadmium to reduce nitrate to nitrite. The nitrite (that originally present plus that reduced to nitrate) is determined by diazotizing with sulfanilamide and coupling with N-(1-naphthyl)-ethylenediamine dihydrochloride to form a highly colored azo dye which is measured colorimetrically. Separate, rather than combined nitrate-nitrite, values are readily obtained by carrying out the procedure first with, and then without, the Cu-Cd reduction step.

This method pertains to the determination of nitrite singly, or nitrite and nitrate combined in surface and saline waters; and domestic and industrial wastes.

Methods for the Determination of Inorganic Substances in Environmental Samples (EPA/600/R-93/100)

(1) Build up of suspended matter in the reduction column will restrict sample flow. Since nitrate-nitrogen is found in a soluble state, the sample may be pre-filtered.(2) High concentrations of iron, copper, or other metals.(3) Large concentrations of oil and grease will coat the surface of the cadmium.

QC Requirements:
Not included.

Maximum Holding Time:
28 Days (nitrate+nitrite)48 hours (nitrate or nitrite, singly)(MCAWW, Table 1).



0.05 - 10 mg/L

Precision and accuracy values were calculated using interlaboratory data from EPA-managed Water Pollution (WP) performance evaluation studies.

The lowest level of detection was taken from the lowest end of the range specified in the method.

Revision Number:
Revision 2.0, August 1993

Test Description:
Nitrate-Nitrate by A

Report Number:

Instrument used for this test:

Analytical testing dots
At least 5 analytes can be tested by ALS - Columbia using EPA Method 353.2. Please contact us for all available analytes which can be tested by EPA Method 353.2.

AnalyteCAS Number
Nitrite as Nitrite14797-65-0
Nitrate+Nitrite as NitrogenCASID10034
Nitrate as Nitrate14797-55-8
Nitrate as Nitrogen14797-55-8A
Nitrite as Nitrogen14797-65-0A

Examples:  8260

Examples:  Dioxin
Analytical testing dots

<-- Search All Test Methods

Suggestions? The test methods page continues to expand and improve. If you have suggestions for improvement, we would enjoy hearing from you. Please contact the webmaster here.

Analytical testing dots