EPA Method 4025
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View Actual EPA Method 4025 (PDF File)
EPA Method 4025:
SCREENING FOR POLYCHLORINATED DIBENZODIOXINS AND POLYCHLORINATED DIBENZOFURANS (PCDD/Fs) BY IMMUNOASSAY
Sample preparation and analysis procedures are described in the manufacturer’s literature. In addition, tests kits are commercially available for this method and the kit instructions used to develop this method.
Method 4025 is a procedure for the analysis of PolyChlorinated DibenzoDioxins and PolyChlorinated DibenzoFurans (PCDD/Fs) in soil at 500 ppt (pg/g). Method 4025 uses an Enzyme Immunoassay (EIA) commercially available test kit containing a polyclonal antibody specific for PCDD/Fs. The EIA kit is designed for the screening of samples according to their toxic equivalent concentration (TEQ) by responding to the toxic PCDD/F congeners in approximate correlation with their toxic equivalency factors (TEFs). The test is capable of multiple congener recognition and preferentially targets congeners with high TEF values; i.e., those with the highest toxicity relative to 2,3,7,8- TetraChloroDibenzo-p-Dioxin (2,3,7,8-TCDD). The final measured EIA response is the sum of the individual congener responses. This response correlates with TEQ because the immunoassay cross-reaction profile for PCDD/Fs correlates with TEF values.
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