EPA Method 4050
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EPA Method 4050:
TNT EXPLOSIVES IN SOIL BY IMMUNOASSAY
Test kits are commercially available for this method. The manufacturer's directions should be followed. In general, the method is performed using an extract of a soil sample. Samples and an enzyme-TNT conjugate reagent are added to a immobilized TNT antibody. The enzyme-TNT conjugate "competes" with TNT present in the sample for binding to the immobilized TNT antibody. The enzyme-TNT conjugate bound to the TNT antibody then catalyzes a colorless substrate to a colored product. The test is interpreted by comparing the color produced by a sample to the response produced by a reference reaction.
Method 4050 is a procedure for screening soil samples to determine when trinitrotoluene (TNT, CAS No. 118-96-7) is present at concentrations above 0.5 mg/kg. Method 4050 provides an estimate for the concentration of TNT by comparison with a reference.
SW-846 Ch 4.4
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