EPA Method 410.4

ALS - Columbia offers EPA Method 410.4 testing at these laboratories:

Jacksonville Jacksonville, Florida Laboratory
Rochester Rochester, New York Laboratory

Method information displayed is provided for informational purposes only. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project). Please contact us for assistance. Analytical testing dots

PDF IconView Actual EPA Method 410.4 (PDF File)

EPA Method 410.4:
Chemical oxygen demand in water by colorimetry. Official Name: Method 410.4: The Determination of Chemical oxygen demand by semi-automated colorimetry

Sample, blanks, and standards in sealed tubes are heated in an oven or block digestor in the presence of dichromate at 150oC. After two hours, the tubes are removed from the oven or digester, cooled, and measured spectrophotometrically at 600 nm. The colorimetric determination may also be performed manuall.Reduced volume versions of this method that use the same reagents and molar ratios are acceptable provided they meet the quality control and performance requirements stated in the method.

This method covers the determination of chemical oxygen demand (COD) in ground and surface waters, domestic and industrial wastes.

Method 410.4: The Determination of Chemical Oxygen Demand by Semi-Automated Colorimetry. Edited by James W. O'Dell. Revision 2.0, August, 1993

Chlorides are quantitatively oxidized by dichromate and represent a positive interference. Mecuric sulfate is added to the digestion tubes to complex the chlorides.Method interferences may be caused by contaminants in the reagent water, reagents, glassware, and other sample processing apparatus that bias analyte response.

QC Requirements:
Demonstration of laboratory capability, and periodic analysis of laboratory reagent blanks, fortified blanks, and other laboratory solutions as a continuing check on performance.

Maximum Holding Time:
28 days




Precision and accuracy values were calculated using interlaboratory data from performance evaluation studies.

MDL is established using reagent water fortified at a concentration of 2 to 3 times the estimated instrument detection limit and is determined from 7 replicate aliquots of the fortified reagent water and processed through the entire analytical method.

Revision Number:
Revision 2.0, August 1993

Test Description:
Chemical Oxygen Dema

Report Number:

Instrument used for this test:

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At least 2 analytes can be tested by ALS - Columbia using EPA Method 410.4. Please contact us for all available analytes which can be tested by EPA Method 410.4.

AnalyteCAS Number
Chemical Oxygen Demand, DissolvedCASID10168
Chemical Oxygen Demand, TotalCASID10313

Examples:  8260

Examples:  Dioxin
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