EPA Method 4430
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View Actual EPA Method 4430 (PDF File)
EPA Method 4430:
SCREENING FOR POLYCHLORINATED DIBENZO-P-DIOXINS AND FURANS (PCDD/Fs) BY ARYL HYDROCARBON-RECEPTOR PCR ASSAY
PCR reagents are added to the microwell and the amount of DRE, which is proportional to the TEQ of the sample, is measured using PCR. The response from the PCR is related to TEQ by a dose-response curve generated from 2,3,7,8-TCDD standards. The PCR can analyze up to 38 samples in duplicate, with blanks, standards and recovery standards simultaneously in 90 minutes.
Method 4430 is a procedure for the screening of Polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) in soil and sediment. This method uses a commercially available Aryl hydrocarbon Receptor (AhR) based polymerase chain reaction (PCR) assay.. The AhR-PCR assay screens samples by their toxicity equivalent quotient (TEQ) by responding to individual PCDD/F congeners in approximate correlation to their toxicity equivalent factors (TEF). The TEQ measured by the AhR-PCR assay is the sum of the response from the individual congeners.
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