EPA Method 535


EPA Method 535 does not directly match ALS - Columbia's list of currently available methods. Please call for availability.


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EPA Method 535:
MEASUREMENT OF CHLOROACETANILIDE AND OTHER ACETAMIDE HERBICIDE DEGRADATES IN DRINKING WATER BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS)

Summary:
Analytes and surrogate are extracted and concentrated by passing a 250-mL water sample through a solid phase extraction (SPE) cartridge containing 0.5 g of nonporous graphitized carbon. The compounds are eluted from the solid phase with a small quantity of methanol containing 10 mM ammonium acetate. The methanol extract is then concentrated to dryness and reconstituted with 1 mL of water containing 5 mM ammonium acetate. The analytes are chromatographically separated by injecting an aqueous aliquot (100 ?L) into an LC system equipped with a reversed phase (C18) column. After elution from the column, the analytes are detected by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The concentration of each identified component is measured by an internal standard procedure, i.e. relating the product ion response of that compound to the product ion response of the compound that is used as an internal standard. A surrogate analyte, whose concentration is known in every sample, is measured with the same internal standard calibration procedure.

Scope:
This is a liquid chromatographic (LC)/tandem mass spectrometric (MS/MS) method applicable to the determination of the ethanesulfonic acid (ESA) and oxanilic acid (OA) degradates of the chloroacetanilide and other acetamide herbicides in finished drinking waters. The single laboratory Lowest Concentration Minimum Reporting Level (LCMRL) has also been determined in reagent water.

Test Description:
Chloroacetanilide and other Acetamide Herbicide Degradates in DW by Solid Phase Extrn and LC/MS/MS

Instrument used for this test:
HPLC-MS

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At least 14 analytes can be tested by ALS - Columbia using EPA Method 535. Please contact us for all available analytes which can be tested by EPA Method 535.

AnalyteCAS Number
Acetochlor ESA187022-11-3
Acetochlor OA194992-44-4
Alachlor ESA142363-53-9
Alachlor OA171262-17-2
Butachlor ESACASID30747
Dimethachlor ESACASID30748
Dimethenamid ESA205939-58-8
Dimethenamid OACASID30667
Flufenacet ESACASID30668
Flufenacet OACASID30669
Metolachlor ESA171118-09-5
Metolachlor OA152019-73-3
Propachlor ESACASID30670
Propachlor OACASID30671





Examples:  8260
TO-15

Examples:  Dioxin
Mercury
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