EPA Method 8082 Testing

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Typically, increased sensitivity of EPA Method 8082 is achieved via three mechanisms:

  • Hardware modifications and/or optimization of the operating conditions of the instrument
  • Use of sample clean-up procedures to eliminate or minimize interferences
  • Introduction of more sample into the instrument

The primary objective is to perform sample preparation techniques that result in reliable, ultra low-level Aroclor quantitation. However, interferences inherent to the sample matrix or introduced during the sample preparation may be enhanced by use of these techniques. If non-target background signal and laboratory contamination can be controlled, significant gains in sensitivity can be achieved.

A relatively large volume or mass of sample is solvent-extracted by the laboratory. After concentration of the sample extract to a small volume, the extract is subjected to Gel Permeation Chromatography (GPC) followed by a large-volume silica gel column cleanup to remove PCB-interfering pesticides and other non-target interferences. These cleanups are followed by concentrated sulfuric acid treatment to remove biogenic interferences.

Further cleanup is performed using elemental mercury to precipitate sulfur, which interferes with the accurate quantitation of Aroclors. Samples are injected into the gas chromatograph using a pressure-programmed LVI that allows the injection of up to 100 µL of sample extract, which essentially results in further concentration of the sample in the instrument. This technique has been used successfully on discharge water, receiving water, sewage influents/effluents, sediment, and tissue. Aroclor reporting limits are 5 ng/L (ppt) for water and 10 µg/kg (ppb) for tissue and sediment. Experimentally-derived detection limits are 2-5 times lower than the reporting limit, depending on the Aroclor. The PCB Congener reporting limits by this method are 5 ng/L (ppt) for water and 0.5 µg/kg (ppb) for tissue and sediment.

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Aroclors - Gas Chromatography (GC), EPA Method 8082A
Method Reporting Limits (MRLs)

Analyte WATER (ng/L)
MRL
SOIL/SEDIMENT (µg/Kg)
(Dry Wt. Basis)
MRL
TISSUE  (µg/Kg)
(Wet Wt. Basis)
MRL
Aroclor 1016 5 10 10
Aroclor 1221 5 10 10
Aroclor 1232 5 10 10
Aroclor 1242 5 10 10
Aroclor 1248 5 10 10
Aroclor 1254 5 10 10
Aroclor 1260 5 10 10
Aroclor 1262 5 10 10
Aroclor 1268 5 10 10

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PCB Congeners - Gas Chromatography (GC), EPA Method 8082A
Method Reporting Limits (MRLs)

 

Analyte PCB Congener WATER (ng/L)
MRL
SOIL/SEDIMENT (µg/Kg)
(Dry Wt. Basis)
MRL
TISSUE  (µg/Kg)
(Wet Wt. Basis)
MRL
PCB 1 2-Monochlorobiphenyl 5 0.5 1
PCB 5 2,3-Dichlorobiphenyl 5 0.5 0.5
PCB 8 2,4'-Dichlorobiphenyl 5 0.5 2
PCB 18 2,2',5-Trichlorobiphenyl 5 0.5 0.5
PCB 28 2,4,4'-Trichlorobiphenyl 5 0.5 0.5
PCB 31 2,4',5-Trichlorobiphenyl 5 0.5 0.5
PCB 33 2',3,4-Trichlorobiphenyl 5 0.5 0.5
PCB 37 3,4,4'-Trichlorobiphenyl 5 0.5 0.5
PCB 44 2,2',3,5'-Tetrachlorobiphenyl 5 0.5 0.5
PCB 49 2,2',4,5'-Trichlorobiphenyl 5 0.5 0.5
PCB 52 2,2',5,5'-Tetrachlorobiphenyl 5 0.5 1
PCB 56 2,3,3',4'-Trichlorobiphenyl 5 0.5 0.5
PCB 60 2,3,4,4'-Tetrachlorobiphenyl 5 0.5 0.5
PCB 66 2,3',4,4'-Tetrachlorobiphenyl 5 0.5 0.5
PCB 70 2,3',4',5-Trichlorobiphenyl 5 0.5 0.5
PCB 74 2,4,4',5-Trichlorobiphenyl 5 0.5 0.5
PCB 77 3,3',4,4'-Tetrachlorobiphenyl 5 0.5 0.5
PCB 81 3,4,4',5-Tetrachlorobiphenyl 5 0.5 0.5
PCB 87 2,2',3,4,5'-Pentachlorobiphenyl 5 0.5 0.5
PCB 90 2,2',3,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 95 2,2',3,5',6-Pentachlorobiphenyl 5 0.5 0.5
PCB 97 2,2',3',4,5-Pentachlorobiphenyl 5 0.5 0.5
PCB 99 2,2',4,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 101 2,2',4,5,5'-Pentachlorobiphenyl 5 0.5 0.5
PCB 105 2,3,3',4,4'-Pentachlorobiphenyl 5 0.5 0.5
PCB 110 2,3,3',4',6-Pentachlorobiphenyl 5 0.5 0.5
PCB 114 2,3,4,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 118 2,3',4,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 119 2,3',4,4',6-Pentachlorobiphenyl 5 0.5 0.5
PCB 123 2',3,4,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 126 3,3',4,4',5-Pentachlorobiphenyl 5 0.5 0.5
PCB 128 2,2',3,3',4,4'-Hexachlorobiphenyl 5 0.5 0.5
PCB 132 2,2',3,3',4,6'-Hexachlorobiphenyl 5 0.5 0.5
PCB 138 2,2',3,4,4',5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 141 2,2',3,4,5,5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 149 2,2',3,4',5',6-Hexachlorobiphenyl 5 0.5 0.5
PCB 151 2,2',3,5,5',6-Hexachlorobiphenyl 5 0.5 0.5
PCB 153 2,2',4,4',5,5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 156 2,3,3',4,4',5-Hexachlorobiphenyl 5 0.5 0.5
PCB 157 2,3,3',4,4',5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 158 2,3,3',4,4',6-Hexachlorobiphenyl 5 0.5 0.5
PCB 166 2,3,4,4',5,6,-Hexachlorobiphenyl 5 0.5 0.5
PCB 167 2,3',4,4',5,5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 168 2,3',4,4',5',6-Hexachlorobiphenyl 5 0.5 0.5
PCB 169 3,3',4,4',5,5'-Hexachlorobiphenyl 5 0.5 0.5
PCB 170 2,2',3,3',4,4',5-Heptachlorobiphenyl 5 0.5 0.5
PCB 174 2,2',3,3',4,5,6'-Heptachlorobiphenyl 5 0.5 0.5
PCB 177 2,2',3,3',4',5,6-Heptachlorobiphenyl 5 0.5 0.5
PCB 180 2,2',3,4,4',5,5'-Heptachlorobiphenyl 5 0.5 0.5
PCB 183 2,2',3,4,4',5',6-Heptachlorobiphenyl 5 0.5 0.5
PCB 184 2,2',3,4,4',6,6'-Heptachlorobiphenyl 5 0.5 0.5
PCB 187 2,2',3,4',5,5',6-Heptachlorobiphenyl 5 0.5 0.5
PCB 189 2,3,3',4,4',5,5'-Heptachlorobiphenyl 5 0.5 0.5
PCB 194 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 5 0.5 0.5
PCB 195 2,2',3,3',4,4',5,6-Octachlorobiphenyl 5 0.5 0.5
PCB 201 2,2',3,3',4,5',6'c-Octachlorobiphenyl 5 0.5 0.5
PCB 203 2,2',3,4,4',5,5',6-Octachlorobiphenyl 5 0.5 0.5
PCB 206 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 5 0.5 0.5
PCB 209 2,2',3,3',4,4',5,5',6,6'Decachlorobiphenyl 5 0.5 0.5

*Please contact your laboratory for the latest limits

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