Abraxis Test Method 500201


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Analytical testing dots

Source:
Abraxis

Method Name:
Pyrethroid by Immunoassay, Magnetic Particle. Official Name: Abraxis Pyrethroid Assay Kit (100T) PN 500201

Description:
Pyrethroid is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.25 mL) and an enzyme conjugate (enzyme-labeled Pyrethroid) are added to a disposable test tube, followed by the addition of paramagnetic particles attached with Pyrethroid-specific antibodies. Both the Pyrethroid in the sample and the enzyme conjugate compete for antibody binding sites on the paramagnetic particles in proportion to their concentrations. At the end of an incubation period, the magnetic particles are separated and washed with the aid of a magnetic separator. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Pyrethroid in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a spectrophotometer, and comparing its absorbance to the absorbance of the calibrators.

Scope:
This method determines Pyrethroids in water (groundwater, surface water, well water). It is capable of detecting Permethrin, Cypermethrin, and other Pyrethroids.

Citation:
Abraxis User Guides and Flowcharts

Interferences:
Cross-reactivity: In addition to Permethrin, the method detects Cypermethrin, and high concentrations Resmethrin, Cyfluthrin, 3-Phenoxy benzoic acid (3-PBA). This method uses Permethrin as calibrators.

QC Requirements:
(A) Calibration with 3 standards and 1 blank, all analyzed in duplicate.(B) Precision: All 46 samples analyzed in duplicate.(C) Accuracy: 3 Matrix samples, spiked with the target analyte at different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if held frozen

Media:
WATER

Subcategory:
Organic

Concentration:
0.75 - 15.0

Precision:
-

Detection:
-

Revision Number:
2005

Analytical testing dots

Related Methods:
Test Method 500001    Test Method 500007    Test Method 500021    Test Method 500061    Test Method 500071    Test Method 500076    Test Method 500081    Test Method 500086    Test Method 500090    Test Method 500201   


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Analytical testing dots