Abraxis Test Method 520011


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Source:
Abraxis

Method Name:
Microcystins and Nodularins by Immunoassay, Microtiter Plate. Official Name: Abraxis Microcystins/Nodularins Plate Assay Kit (96T) PN 520011

Description:
Microcystins and nodularins are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.05 mL) and an antibody against microcystins are added to a microtiter plate well pre-coated with Microcystins-ADDA coating conjugate. Both the microcystins (when present in a sample) and the microcystins coating conjugate compete for the binding sites of the antibody. After another washing step, a second antibody-HRP labeled is added and incubated. After a washing step, a substrate is added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of microcystins in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a plate photometer and comparing its absorbance to the absorbance of the calibrators.

Scope:
The Abraxis Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent (ADDA) detection of microcystins and nodularins in water samples (surface water, groundwater, well water). It is capable of detecting Microcystin-LR, Microcystin-YR, Microcystin-LA, Microcystin-RR, and Nodularins.

Citation:
Abraxis User Guides and Flowcharts

QC Requirements:
(A) Calibration with 4 standards and 1 blank, all analyzed in duplicate.(B) Precision: All 41 samples analyzed in duplicate.(C) Accuracy: 3 Matrix samples spiked with the target analyte at 3 different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if held frozen

Media:
WATER

Subcategory:
Organic

Concentration:
0.10 - 5.0

Precision:
-

Detection:
-

Revision Number:
2002

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Analytical testing dots