Abraxis Test Method 520012

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Method Name:
Microcystins in water by Immunoassay, Coated Tube. Official Name: Abraxis Microcystin Tube Kit (40T) PN 520012

Microcystin is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.50 mL) and an enzyme conjugate (enzyme-labeled Microcystin) are added to a test tube pre-coated with secondary antibodies (anti-rabbit) against Microcystins. Both the Microcystin in the sample (if present) and the enzyme conjugate compete for the limited binding sites on the immobilized anti-rabbit antibodies in proportion to their concentrations. At the end of an incubation period, the unbound molecules are washed and decanted. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Microcystin in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.

This method determines Microcystin in water (groundwater, surface water, well water).

Abraxis User Guides and Flowcharts

Cross-reactivity: Microcystin variants RR and YR and Nodularins and high concentrations of Microcystin-LA produce false positive responses for Microcystin-LR.

QC Requirements:
(A) Calibration with 5 standards and 1 blank, all analyzed in duplicate. (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 5 days with 5 replicates in each of 5 assays. (C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis. (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4 degrees C, longer if frozen



0.10 - 5.0



Revision Number:

Analytical testing dots

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Analytical testing dots