Abraxis Test Method 520012


Method information displayed is provided for informational purposes only.
ALS - Columbia may or may not offer methods shown. Please contact us to learn about ALS - Columbia's latest available methods. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project).

Analytical testing dots

Source:
Abraxis

Method Name:
Microcystins in water by Immunoassay, Coated Tube. Official Name: Abraxis Microcystin Tube Kit (40T) PN 520012

Description:
Microcystin is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.50 mL) and an enzyme conjugate (enzyme-labeled Microcystin) are added to a test tube pre-coated with secondary antibodies (anti-rabbit) against Microcystins. Both the Microcystin in the sample (if present) and the enzyme conjugate compete for the limited binding sites on the immobilized anti-rabbit antibodies in proportion to their concentrations. At the end of an incubation period, the unbound molecules are washed and decanted. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Microcystin in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.

Scope:
This method determines Microcystin in water (groundwater, surface water, well water).

Citation:
Abraxis User Guides and Flowcharts

Interferences:
Cross-reactivity: Microcystin variants RR and YR and Nodularins and high concentrations of Microcystin-LA produce false positive responses for Microcystin-LR.

QC Requirements:
(A) Calibration with 5 standards and 1 blank, all analyzed in duplicate. (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 5 days with 5 replicates in each of 5 assays. (C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis. (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4 degrees C, longer if frozen

Media:
WATER

Subcategory:
Organic

Concentration:
0.10 - 5.0

Precision:
-

Detection:
-

Revision Number:
2007

Analytical testing dots

Related Methods:
Test Method 500001    Test Method 500007    Test Method 500021    Test Method 500061    Test Method 500071    Test Method 500076    Test Method 500081    Test Method 500086    Test Method 500090    Test Method 500201   


<-- Search again



*Method information displayed is provided as a convenience only. No warranty (express or implied) is made as to its accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project).

Suggestions? The test methods page continues to expand and improve. If you have suggestions for improvement, we would enjoy hearing from you. Please contact the webmaster here.


Analytical testing dots