Abraxis Test Method 540021

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Method Name:
Cyclodienes by Immunoassay, Microtiter Plate. Official Name: Abraxis Cyclodienes Plate Assay Kit (96T) PN 540021

Cyclodienes are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.025 mL) and an antibody against Cyclodienes are added to a microtiter plate well pre-coated with Cyclodienes coating conjugate. Both the Cyclodienes (when present in a sample) and the Cyclodienes coating conjugate compete for the binding sites of the antibody. After a washing step, a second antibody-HRP labeled is added and incubated. After a washing step a substrate is added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Cyclodienes in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a plate photometer and comparing its absorbance to the absorbance of the calibrators.

This method determines Cyclodienes in water (groundwater, surface water, well water). It is capable a detecting Dieldrin, Endosulfan, Heptachlor, Aldrin, Chlordane, Toxaphene.

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Cross-reactivity: In addition to Dieldrin, the method detects Endosulfan, Heptachlor, Aldrin, Chlordane, and high concentrations of Toxaphene. The method uses Dieldrin as calibrators.

QC Requirements:
(A) Calibration with 7 standards and 1 blank, all analyzed in duplicate.(B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 3 days with 5 replicates in each of 5 assays.(C) Accuracy: 5 matrix samples, spiked with the target analyte at 3 different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if held frozen



0.10 - 25



Revision Number:

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Analytical testing dots