Abraxis Test Method 54002B

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Method Name:
Benomyl and Carbendazim in water by Immunoassay, Microtiter Plate. Official Name: Abraxis Benomyl/Carbendazim ELISA Plate Kit PN 54002B

Benomyl and Carbendazim are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.10 mL) and enzyme conjugate (Carbendazim-HRP) are added to plate wells pre-coated with antibodies against Benomyl and Carbendazim. Both the Benomyl/Carbendazim (if present) in the sample and Carbendazim-HRP conjugate compete to bind to the limited number of binding sites on the immobilized antibodies in proportion to their concentrations. After incubation, the unbound molecules are washed and decanted. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Benomyl/Carbendazim in the sample is determined by measuring its absorbance at a specific wavelength (450nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.

This method determines Benomyl/Carbendazim in water (groundwater, surface water, well water).

Abraxis User Guides and Flowcharts

Cross-reactivity: Benomyl and high concentrations of Thiabendazole produce false positive responses for Carbendazim.

QC Requirements:
(A) Calibration with 3 standards and 1 blank, all analyzed in duplicate. (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 5 days with 5 replicates in each of 5 assays. (C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis. (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4 degrees C, longer if held frozen.



0.05 - 1.0



Revision Number:

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Analytical testing dots