Abraxis Test Method 540041

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Method Name:
DDE/DDT by Immunoassay, Microtiter Plate. Official Name: Abraxis DDE/DDT Plate Assay Kit (96T) PN 540041

DDE/DDT are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.05 mL) and an antibody against DDE/DDT are added to a microtiter plate well pre-coated with DDE/DDT coating conjugate. Both the DDE/DDT (when present in a sample) and the DDE/DDT coating conjugate compete for the binding sites of the antibody. After a washing step, a second antibody-HRP labeled is added and incubated. After a washing step, a substrate is added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of DDE/DDT in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a plate photometer and comparing its absorbance to the absorbance of the calibrators.

This method determines DDE/DDT in water (groundwater, surface water, well water). It is capable of detecting p,p'-DDE; p,p'-DDD; o,p'-DDD; p,p'-DDT; and, o,p'-DDE.

Abraxis User Guides and Flowcharts

QC Requirements:
(A) Calibration with 6 standards and 1 blank, all analyzed in duplicate.(B) Precision: 3 matrix samples with different levels inthe range for quantitative analysis analyzed daily for 3 days with 5 replicates in each of 5 assays.(C) Accuracy: 5 matrix samples spiked with the target analyte at 3 different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if held frozen



1.25 - 75.0



Revision Number:

Analytical testing dots

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Analytical testing dots