Abraxis Test Method 540041


Method information displayed is provided for informational purposes only.
ALS - Columbia may or may not offer methods shown. Please contact us to learn about ALS - Columbia's latest available methods. No warranty (express or implied) is made as to the website accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project).

Analytical testing dots

Source:
Abraxis

Method Name:
DDE/DDT by Immunoassay, Microtiter Plate. Official Name: Abraxis DDE/DDT Plate Assay Kit (96T) PN 540041

Description:
DDE/DDT are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.05 mL) and an antibody against DDE/DDT are added to a microtiter plate well pre-coated with DDE/DDT coating conjugate. Both the DDE/DDT (when present in a sample) and the DDE/DDT coating conjugate compete for the binding sites of the antibody. After a washing step, a second antibody-HRP labeled is added and incubated. After a washing step, a substrate is added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of DDE/DDT in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a plate photometer and comparing its absorbance to the absorbance of the calibrators.

Scope:
This method determines DDE/DDT in water (groundwater, surface water, well water). It is capable of detecting p,p'-DDE; p,p'-DDD; o,p'-DDD; p,p'-DDT; and, o,p'-DDE.

Citation:
Abraxis User Guides and Flowcharts

QC Requirements:
(A) Calibration with 6 standards and 1 blank, all analyzed in duplicate.(B) Precision: 3 matrix samples with different levels inthe range for quantitative analysis analyzed daily for 3 days with 5 replicates in each of 5 assays.(C) Accuracy: 5 matrix samples spiked with the target analyte at 3 different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if held frozen

Media:
WATER

Subcategory:
Organic

Concentration:
1.25 - 75.0

Precision:
-

Detection:
-

Revision Number:
2003

Analytical testing dots

Related Methods:
Test Method 500001    Test Method 500007    Test Method 500021    Test Method 500061    Test Method 500071    Test Method 500076    Test Method 500081    Test Method 500086    Test Method 500090    Test Method 500201   


<-- Search again



*Method information displayed is provided as a convenience only. No warranty (express or implied) is made as to its accuracy, completeness, or applicability (such as the age of a method and whether or not it applies to your project).

Suggestions? The test methods page continues to expand and improve. If you have suggestions for improvement, we would enjoy hearing from you. Please contact the webmaster here.


Analytical testing dots