Abraxis Test Method 54004B


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Analytical testing dots

Source:
Abraxis

Method Name:
2,4-D in water by Immunoassay, Coated Tube. Official Name: Abraxis 2,4-D Tube Kit (40T) PN 54004B

Description:
2,4-D is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.50 mL) and an enzyme conjugate (enzyme-labeled 2,4-D) are added to a test tube pre-coated with antibodies against 2,4-D. Both the 2,4-D in the samples (if present) and the enzyme conjugate compete for antibody binding sites on the 2,4-D immobilized antibodies in proportion to their concentrations. At the end of an incubation period, the unbound molecules are washed and decanted from the tube. A substrate is added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of dilute acid. The concentration of 2,4-D in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.

Scope:
This method determines 2,4-D in water (groundwater, surface water, well water).

Citation:
Abraxis User Guides and Flowcharts

Interferences:
Cross-reactivity: 2,4-D acid; 2,4-D methyl ester; 2,4-DB; 2,4-D isopropyl ester; 2,4-DB butyl ester; and high concentrations of 2,4,5-T and MCPA produce false positive responses for 2,4-D.

QC Requirements:
(A) Calibration with 3 standards and 1 blank, all analyzed in duplicate (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 5 days with 5 replicates in each of 5 assays (C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4 degrees C, longer if held frozen

Media:
WATER

Subcategory:
Organic

Concentration:
2.0 - 100

Precision:
-

Detection:
-

Revision Number:
2007

Analytical testing dots

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Test Method 500001    Test Method 500007    Test Method 500021    Test Method 500061    Test Method 500071    Test Method 500076    Test Method 500081    Test Method 500086    Test Method 500090    Test Method 500201   


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Analytical testing dots