Abraxis Test Method 580002

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Source:
Abraxis

Method Name:
17Beta-Estradiol by Immunoassay, Magnetic Particle. Official Name: Abraxis 17Beta-Estradiol Assay Kit (100T) PN 580002

Description:
17Beta-Estradiol is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.25 mL) and the paramagnetic particles attached with 17Beta-Estradiol specific antibodies are added to a disposable test tube and allowed to incubate followed by the addition of an enzyme conjugate (enzyme-labeled 17Beta-Estradiol). Both the 17Beta-Estradiol in the sample and the enzyme conjugate compete for antibody binding sites on the paramagnetic particles in proportion to their concentrations. At the end of an incubation period, the magnetic particles are separated and washed with the aid of a magnetic separator. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of 17Beta-Estradiol in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.

Scope:
This method determines 17Beta-Estradiol in water (groundwater, surface water, well water).

Citation:
Abraxis User Guides and Flowcharts

Interferences:
Cross-reactivity: High concentrations of Estrone may produce false positive responses for 17Beta-Estradiol.

QC Requirements:
(A) Calibration with 3 standards and 1 blank, all analyzed in duplicate.(B) Precision: 4 matrix samples with different levels in the range for quatitative analysis analyzed daily for 3 days with 5 replicates in each of 5 assays.(C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis.(D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.

Maximum Holding Time:
14 days at 4oC, longer if frozen

Media:
WATER

Subcategory:
Organic

Concentration:
1.5 - 25.0

Precision:
-

Detection:
-

Revision Number:
2001



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Test Method 500001    Test Method 500007    Test Method 500021    Test Method 500061    Test Method 500071    Test Method 500076    Test Method 500081    Test Method 500086    Test Method 500090    Test Method 500201   

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