AOAC Test Method 991.08

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Method Name:
Glyphosate and AMPA in Environmental Water. Official Name: Glyphosate and Aminomethylphosphonic Acid (AMPA) in Environmental Water by Liquid Chromatographic Method

Environmental water containing glyphosate and aminomethylphosphonic acid (AMPA) is evaporated to dryness using a rotary evaporator and the residue is dissolved in EDTA solution. Glyphosate and AMPA are separated and detected by liquid chromatography using a postcolumn reaction specific for primary amines. Glyphosate is oxidized in a postcolumn reactor coil at 50 degrees C with calcium hypochlorite to form glycine. Glycine is reacted with o-phthalaldehyde (OPA) in the presence of mercaptoethanol (MERC) in a second coil to form a fluorophor, which is detected fluorometrically (excitation 340 nm, emission 425-455 nm). AMPA is relatively unreactive toward calcium hypochlorite but undergoes a similar reaction with OPA-MERC reagent to form another fluorophor, which is detected fluorometrically under same conditions as for glycine.

Applicable to determination of glyphosate and AMPA in ground water, drinking water, and surface water at 0.5-5000 ug/L.

Official Methods of Analysis of AOAC International, 16th Edition, 4th Revision, 1998 Volume I

Method interferences caused by contaminants in solvents, reagents, glassware, and other sample-processing hardware that lead to discrete artifacts and/or elevated base lines in chromatograms.

QC Requirements:
IDMP, MB, SS, CV (every 2 samples)



0.5 - 5000ug/L


Lowest level from calibration range of the method.

Revision Number:
Final Action - 1993

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