AOAC Test Method 991.08


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Source:
AOAC

Method Name:
Glyphosate and AMPA in Environmental Water. Official Name: Glyphosate and Aminomethylphosphonic Acid (AMPA) in Environmental Water by Liquid Chromatographic Method

Description:
Environmental water containing glyphosate and aminomethylphosphonic acid (AMPA) is evaporated to dryness using a rotary evaporator and the residue is dissolved in EDTA solution. Glyphosate and AMPA are separated and detected by liquid chromatography using a postcolumn reaction specific for primary amines. Glyphosate is oxidized in a postcolumn reactor coil at 50 degrees C with calcium hypochlorite to form glycine. Glycine is reacted with o-phthalaldehyde (OPA) in the presence of mercaptoethanol (MERC) in a second coil to form a fluorophor, which is detected fluorometrically (excitation 340 nm, emission 425-455 nm). AMPA is relatively unreactive toward calcium hypochlorite but undergoes a similar reaction with OPA-MERC reagent to form another fluorophor, which is detected fluorometrically under same conditions as for glycine.

Scope:
Applicable to determination of glyphosate and AMPA in ground water, drinking water, and surface water at 0.5-5000 ug/L.

Citation:
Official Methods of Analysis of AOAC International, 16th Edition, 4th Revision, 1998 Volume I

Interferences:
Method interferences caused by contaminants in solvents, reagents, glassware, and other sample-processing hardware that lead to discrete artifacts and/or elevated base lines in chromatograms.

QC Requirements:
IDMP, MB, SS, CV (every 2 samples)

Media:
WATER

Subcategory:
Organic

Concentration:
0.5 - 5000ug/L

Precision:
-

Detection:
Lowest level from calibration range of the method.

Revision Number:
Final Action - 1993

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Analytical testing dots